Electrophorectic mobility shift assay (EMSA), gel-shift, band-shift is used to study what?Outline…
- SCIENCE
Describe how a chain termination sequencing reaction works, and how the products of the reaction…
In chain termination sequencing we synthesize new strands of DNA which are complementary to a single-stranded template. The template DNA is provided with a mixture consisting of all four…
- SCIENCE
Electrophorectic mobility shift assay (EMSA), gel-shift, band-shift is used to study what?Outline…
Electrophoretic Mobility Shift Assay or ASMA is also known as gel shift assay, band shift assay or gel retardation assay. This is a very well used technique for studying interactions between DNA-…
- SCIENCE
Outline how a cDNA library is prepared and screened to identify the desired clone.In…
Cloning an entire genome in the form of a library of random genomic clones is often called a shotgun experiment. In this experiment, genomic DNA is extracted, broken into fragments of reasonable…
- MACBETH
Who was betrayed in Shakespeare’s Macbeth and why?Name the characters and who betrayed them and…
Betrayal is a huge part of Macbeth. The play opens with a battle that is being fought because two of the Thanes (Lords) who are supposed to be serving King Duncan have turned against him. In the…
1 educator answer
- RIP VAN WINKLE
What are the symbolic meanings of the bearded men with odd appearance in the Catskill Mountains…
Although Henry Hudson was an English explorer, he worked for the Dutch East India Company, so when he explored the New York river valley that was later named after him (the Hudson River, and the…
1 educator answer
- MATH
Universal Set={whole numbers} A={multiples of 5} B={multiples of 3} C={multiples of 10} Write…
Here we take only postive numbers. U = {…, 0 ,1, 2, 3….} whole numbers A = {….,0, 5, 10, 15,…..} B= {…,0, 3, 6, 9,18,….} C = {….,10,20,30,…..} Solution: A ={x : x= 5n } for n =…
- LOIS LOWRY
What is the main theme in Messenger by Lois Lowry?
To me, the main theme in this book is how greed can cause hatred and intolerance. At the start of the book, more or less everything is okay. The people of the village are so welcoming, so into…
2 educator answers
- A ROSE FOR EMILY
Do you think the pressure and judgment of her local community pushed Emily to a breaking point in…
There seems to be very little textual evidence in “A Rose for Emily” to support a conjecture about Emily’s reaction to the community or as to whether she perceived them as pressuring and judging…
10 educator answers
- THE LOVE SONG OF J. ALFRED PRUFROCK
How does Prufrock differentiate himself from Hamlet ?
By saying that he is not Prince Hamlet and was not meant to be Hamlet, Prufrock is, of course, making us think of how he is, in his indecision, similar to Hamlet, as in the experiment in which…
1 educator answer
- THE ADVENTURES OF HUCKLEBERRY FINN
“Keep away, boy – Keep to looard” Ch.16, P 75. “looard ?” Please explain to me.
The way Twain writes this word (in dialect) makes it very hard to understand what the word would be in proper English. In proper English, the word is “leeward.” This word means “on the downwind…
1 educator answer
- LAW AND POLITICS
What is a treaty of double taxation?
In a way, this sort of a treaty would make more sense if it were called a treaty against double taxation. This is much more like what the treaty actually does. What such a treaty does is to make…
1 educator answer
- RAY BRADBURY
What are examples of irony in “A Sound of Thunder”?
The most re-published short story in science fiction history, Ray Bradbury’s “A Sound of Thunder” deals with the idea of time travel and how subtle changes in the past can drastically affect the…
1 educator answer
- SCIENCE
What is the difference between premise and hypothesis?
As used in field of science premise refers to some thing that is assumed to be true and then used to draw conclusion. In such reasoning no claim may be made about the truth or validity of the…
- SCIENCE
How can I see my teacher’s pupils dilate without any instruments?
The pupil is the hole located at the center of the iris (the colored part of your eye) that allows light to enter the retina (tissue lining the inner surface of the retina). Muscles attached to…
2 educator answers
- SCIENCE
What is the difference between a hormone and an enzyme
i need to add one more point to the above answer that hormones are secreted by ductless glands while enzymes are secreted by glands having ducts.
- THE GREAT GATSBY
How does the theme “superficial relationships” relate to the novel “The Great Gatsby”?please use…
Most of the relationships in the novel are superficial; that is, they lack depth of feeling and are based on surface qualities only. Daisy and Tom are married because it was expected of both of…
1 educator answer
- THROUGH THE TUNNEL
Resolution/denouenment for “Through the Tunnel?”
The resolution of Doris Lessing’s “Through the Tunnel” occurs after Jerry makes it through the tunnel alive and resurfaces above the water on the other side of the huge rock. At this point, the…
1 educator answer
- ROBINSON CRUSOE
how can we analyse pantomime of walcott derek?Compare Robinson crusoe and pantomime of walcott…
his name is derek walcott
- ROMANTICISM
How does Henry Wadsworth Longfellow poem “The Cross of Snow” and the “Psalm of Life” relate to…
- Both poems are didactic, meaning that they strive to teach the reader something. For the Romantics, it was important that their works featured morals and lessons. In “Psalm,” Longfellow…
1 educator answer
- THE RIME OF THE ANCIENT MARINER
‘He prayth best who loveth best’ – explain clearly.
Let’s see the full idea, which comes at the end of Coleridge’s poem about a man possessed: “…He prayeth well, who loveth wellBoth man and bird and beast.He prayeth best, who loveth bestAll…
1 educator answer
- THE MINISTER’S BLACK VEIL
How does The Minister’s Black Veil relate to the Romantic’s time period?
There are two areas where I can find a connection between “The Minister’s Black Veil” and American Romanticism. One is the importance of individual freedom in the sense that each person has the…
2 educator answers
- HISTORY
Why did Russia sell Alaska to the USA?
Not surprisingly, the major reason that Russia sold Alaska to the United States was the fact that the Russian Tsar needed money. Russia sold Alaska to the United States in 1867. The Tsar at the…
1 educator answer
- MATH
Who discovered complex numbers?
Complex number is a number that consists of two components a real number component and an imaginary number component. A complex number is represented as: a + bi Where ‘a’ represents the real number…
- ARABY
What is the most important element of fiction in this story?I need help, I am not sure what is…
This story features a crushing moment of epiphany, which is when a character realises a central truth about themselves and their life. We are introduced to the narrator who, in a childlike way,…
5 educator answers
- MATH
What is the value of the derivative of cosec ( x^2+3) at x equal to 2
To find the derivative of cosec(x^2+3) at x= 2. Solution: We know that d/dx(u(v(x) =(du/dv)(dv/dx). Let x^2+3 = t. Then cosec (2x^2+3 ) = cosect. we know cosec t = 1/sint. Therefore (Cosec t)’ =…
- THE ROAD
How is the boy a character foil to his father in The Road?
A character foil is a character who highlights the hidden or opposing traits in the main character. In McCarthy’s The Road, the boy is a foil for his father because the boy highlights the lost…
2 educator answers
- SCIENCE
What is the purpose of adrenaline.
Adrenaline plays the role of a very important neurotransmitter. In addition, adrenaline when released into the circulatory system, has effects on all the major organs in our body. This happens when…
- A POISON TREE
What difference would it make if it were a peach in an orchard? What does the apple is the garden…
I would consider that Blake chose to use the idea of the apple tree to contextualise the poem as a religious sermon – a former title of the text was Christian Forbearance’ The apple symbol fits…
1 educator answer
- MATH
Find the area under the standard normal curve between +1.50 standard deviations and +2.50standard…
The area of the standard normal curve betwee 1.50standard devition and 2.5 standard deviation (from the mean) is the area under the normal curve given by: Integral 1/sqrt(2pi)}{ e^-(0.5x^2)}dx…
- LITERATURE
In Atlas Shrugged, what does the title signify?
The title of the book is best explained by a conversation between Francisco and Hank Rearden. Below is a quotation within Atlas Shrugged from that discussion(Part 1, page 422). “Mr. Rearden,” said…
- SCIENCE
Why are there more lunar eclipses than solar eclipses?
That’s a cool question, and it has a cool answer. It all has to do with sizes and distances. The moon is about 240,000 miles from the earth. That sounds like a long distance for sure. But…
1 educator answer
- SCIENCE
Who created the first computer virus?
There have been many programs that resemble computer viruses, but the first one that can be truly called a Virus as it spread in the wild was one called Elk Cloner which could spread using the…
- SCIENCE
Does the period of oscillation of a string remain the same irrespective of the weight that is hung?
The period of oscillation of a simple pendulum depends only on the length of the pendulum and the acceleration due to gravity. In this case it is assumed that the string has no weight. The weight…
- FAHRENHEIT 451
what part did the river play in montag’s escape?
The river is what takes him away from the surreal world that he was living in and into a reality that is more real; like a rebirthing: ” He felt as if he had left a stage behind and many…
- BLACK BOY: A RECORD OF CHILDHOOD AND YOUTH
What is the significance of the opening and closing scene in the novel Black Boy?
The opening and ending scenes of the autobiography Black Boy (American Hunger) show Richard’s independent personality and his rejection of every type of authority. In the opening scene, it is the…
1 educator answer
- SCIENCE
At what angle does a missile have to be launched to cover the greatest distance.
For an object thrown with a speed v and at an angle T with respect to the horizontal the distance of its trajectory is given by the equation: D = [( v^2 *(sin 2T) / g]. If we want to make D the…
- MATH
Solve for x and y. f’(x)= 7x+ 5 , x+3y =6
To solve f'(x) = 7x+5….(1) and x+3y = 6 …………………(2). Solution: We presume y = f(x). Then from the 2nd equation x+3y =6, we get: 3y = 6-x y =(6-x)/3 y = 2-(1/3)x. y’ = {(2-(1/3)x}’ =…
- NIGHT
What is the significance of Juliek’s last concert?
Juliek’s “last concert” happens just after the prisoners are forced to physically run from Buna to Gliewitz in the snow. This is an example of one of the many “death marches” that took place…
1 educator answer
- SCIENCE
Does the International Date Line pass through Greenwich?
No, the International Date Line does not pass through Greenwich, England. The International Date Line is only in the Pacific Ocean, on more or less the exact opposite side of the world from…
2 educator answers
- MATH
If the perimeter of a triangle is equal to its area and one of the sides is 6, what are the…
Given the perimeter of the triangle = sum of the sides= a+b+c. Therefore by data a+b+c = A. Also a = 6, where a,b,c are the sides of a triangle. Solution: For simlicity we assume b=c =x, say. Then…
- LITERATURE
what is the problem in The Egypt Game and the resolution?
There are several mysteries in The Egypt Game the biggest one being what is the role of “The Professor” the strange, reclusive man who owns the yard where the kids play the game. He is suspected by…
- MATH
What are the sides of a right angled isosceles triangle?
Let abc be an isoscele right angle triangle, such that: ab = bc angle b = 90 Since ab = bc , then angle a = angle c But angle a + angle c = 90 ==> angle a = angle c = 45 We know that: cosa =…
1 educator answer
- ARTS
Whats happening???I dont understand anymore. Enotes questions arent answerd at all anymore. Less…
I’m not sure I have the answer you are looking for, but I will say many questions are still being answered almost immediately. Often, questions which go unanswered for a long time are either…
3 educator answers
- THE LESSON
How do the elements of this short story contribute to your understanding of Sylvia’s learning of…
The reader understands that Sylvia learns a lesson in Bambara’s “The Lesson” because of the ambiguity that Bambara uses in the story. When Miss Moore takes the children to F. A. O. Schwartz to…
1 educator answer
- SCIENCE
A 0.49 kg softball is pitched at a speed of 18 m/s.The batter hits it back directly at the…
The initial velocity of the ball is 18 m/s moving away from the pitcher and towards the batter. The batter strikes the ball and its velocity changes direction and is now moving at 23 m/s towards…
- THE PILGRIM’S PROGRESS
What are the morals in The Pilgrim’s Progress?
Allegory: The term loosely describes any writing in verse or prose that has a double meaning. This narrative acts as an extended metaphor in which persons, abstract ideas, or events represent not…
1 educator answer
- HISTORY
Is there any hope that Harappan script might someday be read? ‘Earliest writing’ found…
According to the scholar who the article quotes (Dr. Meadow), there is not much hope at this point. But you have to figure that there are always poss
- HISTORY
ibilities. To me, the main possible way to…
1 educator answer
- HISTORY
Why can’t the Harappan script be read? ‘Earliest writing’ found…
According to the article, the Harappan script can’t be read for two reasons. First, Harappan did not give rise to any later languages. Since it did not, we have no related languages to use while…
1 educator answer
- LORD OF THE FLIES
When Ralph finally begins to sob uncontrollably, what is he weeping for and what has he…
(Please ask only one question at a time on the eNotes site.) When Ralph begins to sob uncontrollably, it comes from a variety of emotions. He is no doubt relieved to be rescued at the nick of time,…
1 educator answer
- THE LOTTERY
Explain the possible allusions or symbolism of the characters names.Delacroix Graves Summers…
Each of the characters you listed could be considered an example of symbolism, some of which are very obvious. For example, Mr. Summers and Mr. Graves have surnames that are even symbolic; they…